Association of FCgamma RIIA polymorphism with brucellosis

Abstract number: 1733_312

Rafiei A., Ghasemian R., Hosseini khah Z., Ajami A.

Background and Purpose: Brucellosis is a major bacterial zoonosis of global importance. Effective host defence against brucella depends on immunoglobulin G-mediated phagocytosis of the bacteria and it has been shown that the FC RIIA polymorphism (FCgamma RIIA-R131 vs FCgamma RIIA –H131) determines the capacity of immunoglobulin G-mediated phagocytosis via this receptor. The aim of the study was evaluation of FCgamma RIIA polymorphism in patients with brucellosis and reveals any relation between this polymorphism and disease progression.

Materials and Methods: In this study we evaluated FCgamma RIIA polymorphisms (R/R131, R/H131, H/H131) in 67 patients with serologically proven brucellosis and 67 age, gender and geographical healthy volunteers. FCgamma RIIA polymorphism was determined using a polymerase chain reaction (SSCP-PCR).

Results: The frequency of FCgamma RIIA-R/R131 genotype was higher in patients with brucellosis compared Controls (47.8% vs 28.4%). This genotype has an OR = 2.1, 95%CI = 1.3–4.2, P = 0.039) Significant correlation with brucellosis. But we did not find a significant difference between patients with brucellosis and controls (P = 0.2).

Although the frequency of FCgamma RIIA-R/R131 was higher in patients with brucellosis compared controls, we did not find any statistically differences (53.8% vs46.3%, P = 0.2). As a result there was no significant association between FCgamma RIIA genotype and severity of brucellosis.