Essential oil composition and antibacterial effects of Ziziphora clinopodioides

Abstract number: 1733_303

Chitsaz M., Barton M., Bazargan M., Kamalinejad M.

Background: The plant of Ziziphora clinopodioides (LAM), which is habitant of Iran, has been used in Iranian Traditional Medicine for treatment of some infectious conditions. In this survey, its antibacterial effects were examined against some bacterial species.

Methods: Hydro-alcoholic extract of plant obtained by solvent (methanol 70%) and essential oil obtained by hydro-distillation procedure. The methanolic extract and essential oil were tested against Staphylococcus aureus (ATCC25923), Streptococcus pyogenes (PTCC1470), Escherichia coli (ATCC25922), Salmonella typhimurium (PTCC1609), Klebsiella pneumoniae (PTCC1053), and Pseudomonas aeruginosa (ATCC27853). Standard agar diffusion method was used for antimicrobial assay of methanolic extract and essential oil. MIC and MBC were determined by using macrobroth dilution method. Compounds of essential oil separated and identified by GC and GC/MS analysis.

Results: The growth of Gram-positive organisms (S. aureus and S. pyogenes) were inhibited by methanolic extract at a concentration of 25 mg/well, but it did not inhibit any of the tested Gram-negative species. Essential oil halted the growth of all tested Gram-positive and Gram-negative organisms, with highest effect on S. typhimurium (with MIC and MBC of 225 micg/mL). Gas chromatographic analysis revealed 22 different compounds in essential oil which five of them comprise more than 73% of oil and pulegon is the highest one.

Conclusion: The results suggest that Ziziphora clinopodioides has potent antibacterial effects on some Gram-positive and Gram-negative bacterial species especially on Salmonella typhimurium.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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