Eradication of induced mouse fibrosarcoma with combination of verotoxin1 and monophosphoryl lipid A

Abstract number: 1733_299

Hosain Zadegan H., Sattari M., Mohammad Hasan Z., Allameh A.

Objectives: Because of disadvantages of routine cancer therapies such as chemotherapy and radiotherapy, four essential approaches of different microbial metabolites have been used recently in treatment of cancers. These are enterotoxins, bacterial proteins, recombinant toxins, and combination therapies. Antitumour effects of verotoxin1 have known from several years ago, that produced from some kinds of Escherichia coli. Our purpose was to study the synergistic cytotoxicity of verotoxin1 and monophosphoryl lipidA (MPL) a nontoxic derivative of lipopolysaccharide on induced fibrosarcoma of Balb/c.

Methods: For determining treatment doses of Verotoxin1 and MPL on animals, lethal dose 50 percent of them calculated with Reed & Munch method. Then intradermal tumours (mean diameter of 0/7–1 centimeter) induced with WEHI-164 cells on back of animals, and divided in four groups. Group 1 used as control, and groups 2, 3, 4 injected with MPL, verotoxin1, and combinations of MPL and verotoxin1 respectively. After treatment, eradication or decrease in volume of tumours monitored daily and compared in 4 groups.

Results: verotoxin1 like standard vero cell line indicated cytotoxicity on WEHI-164 line in In vitro experiments. Simultaneous treatment of tumours with verotoxin1 & MPL eradicated malignant fibrosarcoms of balb/c. Animals of control group died after severe weight loss in comparison.

Conclusion: Our results indicate that verotoxin1 have synergistic effects with MPL in eradication of malignant fibrosarcomas of Balb/c. And after further studies, it can be used for treatment of different tumours. Application of tow microbial metabolite as a treatment of tumours proposed and studies firstly in this study. On the other hand results indicate that tow factors have synergy in pathogenesis of verotoxigenic E. coli infections.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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