Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates from ocular infections

Abstract number: 1733_247

Maraki S., Nioti E., Georgiladakis A., Gitti Z., Neonakis I., Katrinaki M., Tselentis Y.

Objective: To determine the antimicrobial susceptibilities and serotypes of Streptococcus pneumoniae recovered from ocular and periocular infections from 1997 through 2006 in the University Hospital of Crete, Greece.

Material and Methods: A total of ninety Streptococcus pneumoniae isolates were studied. Pneumococci were identified by using standard techniques, including Gram stain characteristics, colonial morphology, optochin susceptibility, and bile solubility. Susceptibility tests were done by the E-test method according to manufacturer's recommendations and were interpreted according to the CLSI guidelines. The following antibiotics were tested: penicillin G, cefuroxime, cefotaxime, ceftriaxone, cefepime, imipenem, meropenem, erythromycin, clarithromycin, clindamycin, ciprofloxacin, levofloxacin, sparfloxacin, moxifloxacin, chloramphenicol, tetracycline, trimethoprim/sulfamethoxazole, and vancomycin. Serotyping was performed by the capsular swelling method with specific antisera.

Results: Fourteen isolates (15.5%) showed intermediate resistance and 7 (7.8%) high-level resistance to penicillin. Erythromycin, clarithromycin, clindamycin, chloramphenicol, tetracycline, and trimethoprim/sulfamethoxazole resistance rates were 32.2, 32.2, 14.4, 1.1, 25.6 and 15.6%, respectively. All isolates were sensitive to vancomycin and to all 4 quinolones tested. The most prevalent serotype was 19, followed by 6, 9 and 14.

Conclusion: The increased resistance rates of S. pneumoniae to penicillin, macrolides and other antibiotics indicate the importance of performing antimicrobial susceptibility testing in order to determine the appropriate therapy. Fluoroquinolones are highly active in vitro against ocular pneumococcal isolates including penicillin resistant strains and may offer enhanced coverage for these organisms.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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