In vitro activity of fosfomycin tromethamine and linezolid against clinical vancomycin-resistant Enterococcus faecium isolates

Abstract number: 1733_194

Cilli F., Pullukcu H., Aydemir S., Sipahi O., Tasbakan M., Turhan A., Ulusoy S.

Objectives: Infections with vancomycin resistant enterococci are emerging problems. The aim of this study was investigate the in vitro effect of fosfomycin tromathanole and linezolid against 117 vancomycin-resistant isolates of Enterococcus faecium.

Method: MIC of fosfomycin and linezolid were determined by E test (Biodisk, Sweden). A 0.5 McFarland suspension of the microorganism in 0.9% saline was inoculated into Mueller-Hinton agar (Oxoid, England). E test strips were placed on the culture plates and the MIC read after 24 h. Since E test strips for fosfomycin contained glucose-6-phosphate, extra supplementation of the compound in the culture medium was not done. The readings were tabulated and the MIC50 and MIC90 values determined. The breakpoint criteria to determine susceptibility were based on the CLSI. All isolates were Enterococcus faecium (89 from rectal swabs, 24 from hemoculture, 3 from tissue biopsy culture, 1 from urine culture).

Results: The MIC90 and MIC50 of fosfomycin were 192 mg/L and 512 mg/L and for linezolid 1 mg/L and 2 mg/L, respectively. Overall MIC for linezolid ranged between 0.5–3 mg/dl.

Conclusion: Linezolid but not fosfomycin tromethanol had good in vitro activity against 117 isolates of vancomycin resistant E. faecium.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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