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Slime production in blood isolates of Staphylococcus aureus under various growth conditions

Abstract number: 1733_114

Aslan O., Aksu B., Babacan F.

Objectives: The production of biofilm represents an important virulence factor of certain strains of Staphylococcus aureus. We aimed to investigate biofilm production of blood-borne S. aureus isolates under different growth conditions.

Methods: Total of 100 blood isolates of S. aureus were included to the study. All the isolates were identified as S. aureus by colony morphology, catalase and tube coagulase reactivity. Slime production of the isolates was detected by cultivation on Congo Red Agar (CRA) plates and quantitative microplate test. Four different Tryptic Soy Broth (TSB) formulas were used in microplates for testing their influence on biofilm production; standard TSB, TSB supplemented with 1% glucose, iron limited TSB (50% less than usual) and iron supplemented TSB (50% more than usual).

Results: Only one isolate was detected as positive with three methods; CRA and quantitative microplate method with standard TSB and iron limited TSB. Of one hundred isolates, 8 were found positive (8%) in glucose supplemented TSB medium which were also found positive in iron limited TSB medium. In addition to these 8 isolates, 37 isolates (45%) were also determined as positive in iron limited medium, but only one isolate (1%) was detected as positive in iron supplemented TSB.

Conclusion: Iron limited TSB medium might be the best approach to evaluate biofilm production of clinical S. aureus isolates.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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