Bacterial biofilms in patients with chronic rhinosinusitis
Abstract number: 1733_111
Dworniczek E., Fraczek M., Kassner J., Adamski R., Seniuk A., Choroszy-Król I.
Objectives: Microbial biofilms that are formed on human tissue surfaces play a role in many chronic diseases. Existing in a biofilm phenotype microorganisms evade host defences and are resistant to systemic and local antibiotic therapy. Biofilms have been implicated in dental and periodontal diseases, chronic tonsilitis, otitis media. We demonstrated that bacterial biofilms are present in patients with chronic rhinosinusitis (CRS). Although many etiological factors contributing to CRS have been described, the role of bacteria is not well definied.
Methods: We reviewed 9 cases of CRS patients using culturing methods, scanning (SEM) and transmission (SEM) electron microscopes. The patients were undergoing functional endoscopic sinus surgery (FESS) or radical antrostomy performed because of failure of past medical therapy. Mucosal specimens and sinus lavage were taken from diseased maxillary sinuses and cultured on media for aerobic and anaerobic bacteria and fungi.
Microrganisms were identified by conventional biochemical tests. The samples were prepared using standard methods for SEM and TEM. Areas of interest were photographed.
Results: Bacterial biofilms observed under electron microscope were detected in 3 patients. Cultures of these specimens contained: Streptococcus spp., Escherichia coli, Klebsiella pneumoniae, Streptococcus agalactiae, Proteus mirabilis. In 2 patients neither bacteria nor fungi were present (negative culture and microscopy). In the samples of 4 patients Gram-negative rods, alpha-haemolytic streptococci, Propionibacterium spp. and Corynebacterium spp. were identified. However no biofilm-like structures were observed under electron microscope.
Conclusion: Biofilms were demonstrated to be present in patients undergoing surgery for CRS. This is one of not numerous documentations of biofilms in association to chronic rhinosinusitis.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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