Use of avian influenza genodiagnostics in the Republic of Kazakhstan
Abstract number: 1733_99
Mizanbayeva S.U., Ospanov K.S., Yingst S.L., García-Sastre A., Favorov M.O., Kazakov S.V., Zeman V.V.
A case of mass wild bird die-off was recorded in March 2006 at the Caspian seacoast close to Aktau, the Mangustau Oblast seat, West Kazakhstan. As a disease control measure, a number of samples were collected to investigate the presence of influenza A virus H5 subtype.
Objectives: Determine the possible presence of influenza A virus H5 subtype in:
1. Samples obtained from patients who could have had physical contact with wild birds and were hospitalised at the Aktau infectious disease hospital with the flu, pneumonia or acute respiratory viral infection symptoms; and
2. Samples obtained from dead wild birds found in the vicinity of Aktau.
Methods: An RT-PCR method was used for the detection of the influenza A virus H5 subtype RNA. The reaction protocol and Flu H5+ 1456 and Flu H51685 primers used in the study were provided by NAMRU-3 laboratory, Egypt, Cairo. For verification and detection of the influenza A virus RNA, and concurrent identification of the H5 subtype, we used the AmpliSens Influenza Virus A-H5/H7 test system provided by the Epidemiological Research Institute, Moscow.
- 12 samples obtained from the tracheas, lungs, intestines, and brains of 3 dead swans;
- 1 blood sample obtained from a live swan;
- 4 nasopharyngeal swabs obtained from patients presenting symptoms of acute respiratory viral infection or flu;
- 16 blood serum samples obtained from patients hospitalised in the pulmînology unit of the Aktau infectious-disease hospital.
Results: The RT-PCR method (based on the use of the NAMRU-3 lab, Cairo, Egypt primers) was negative for influenza A virus H5 subtype in the 16 blood serum samples and 4 nasopharyngeal swabs obtained from patients. RNA from influenza A virus H5 subtype was found in the samples obtained from tracheas, lungs, and intestines of all three birds (swans). Identical results were obtained using the NAMRU-3 lab, Cairo, Egypt primers and the test system provided by the Epidemiological Research Institute, Moscow.
Conclusion: Owing to the RT-PCR method we were able to detect the presence of the influenza A virus H5 subtype among the wild birds in the Mangustau Oblast, the Republic of Kazakhstan.
The results obtained using the NAMRU-3 lab, Cairo, Egypt primers and the AmpliSens Influenza Virus A-H5/H7 test system provided by the Epidemiological Research Institute, Moscow were 100% identical. (This project was supported by the US Department of Defense, Biological Threat Reduction Program, Project #KZ-27.)
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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