Investigation of Toxocara canis antibodies in patients with eosinophilia and comparison of two methods: ELISA and Western Blot

Abstract number: 1733_38

Öner Y.A., Artinyan E.

Objectives: Eosinophylic cells, which are only present in gastrointestinal mucosa and form a very little part of peripheric leucosites, the blood levels are controlled districtly in healthy individuals. However, in many diseases (allergic diseases, parasitic infections and cancer), the count of eosinophylic cells increase meaningfully. Especially in parasitic infections the blood counts of these cells can reach 50,000 cells/mL. Larva migrans (visceral, ocular, cutaneous), in which eosinophilia is a constant finding and is transmitted by pets to humans, is described as the migration of Toxocara spp. larvas to the internal organs, eye or dermal tissues with pathological damage. Almost in all articles and books the primary factor of the disease is mentioned to be Toxocara canis.

Methods: The most prefered method in serological diagnosis is to detect specific anti-Toxocara antibodies. If these antibodies are found to be positive then it is suggested to use Western Blot technique in the distinctive diagnosis of the illness. In our study, in a study group patients with eosinophilia, we compared ELISA and Western Blot methods which is found to be more specific and sensitive than ELISA.

Results: In 92 sera, 25 of them were evaluated as positive (27.2%), five of them were evaluated as equivocal (5.4%) and finally 62 (65.3%) of them were found to be negative with ELISA method. On the other hand, same 92 sera were studied with Western Blot technique; 45 of them were found to be positive with the molecular weight bands of 24, 28, 30 and 35 kDa and 47 of them were evaluated as negative while no anti-Toxocara antibody specific molecular weight bands were formed on the strips.

Conclusion: According to these data; when eosinophilia having blood counts are encountered, the diagnosis probability of Toxocariasis is very high. Moreover, our study indicated once more that in serological diagnosis of Toxocariasis, Western Blot method is a more valuable method with its high specificity and sensitivity than ELISA method.

Test resultsSerum numbers (n)%
ELISA(+) WB(+)2325
ELISA(-) WB(+)1718.5
ELISA(?) WB(+)55.4
ELISA(+) WB(-)22.2
ELISA(-) WB(-)4548.9

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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