The prevalence of Dientamoeba fragilis infection in patients with suspected enteroparasitic disease in Denmark

Abstract number: 1733_24

Stensvold C.R., Arendrup M.C., Mølbak K., Nielsen H.V.

A study was taken on to describe the prevalence of D. fragilis in patients with suspected enteroparasitic disease in Denmark and the diagnostic relevance of examining preserved rather than unpreserved stool samples. Parasitological examination of paired stool samples from 103 patients was completed using two different techniques: A formol ethyl-acetate concentration technique (FECT) on unpreserved faeces and a permanent staining technique (PST) on faeces preserved with sodium acetate-acetic acid-formalin (SAF). Using SAF-PST and FECT, 25% and 15% of the specimens were parasite-positive, respectively. D. fragilis was detected only in SAF-preserved stools, and 12/103 (12%) patients were shown to harbour the parasite, only two of which were shown also to host other traditionally acknowledged pathogenic parasites. The present study shows that D. fragilis is remarkably prevalent in Denmark. It confirms the relevance of examining SAF-fixed stools of patients with suspected intestinal parasitosis in settings where the examination of freshly passed, warm stools is not an option, since at least 8/10 cases of potentially symptomatic intestinal parasitosis go undetected using conventional concentration and microscopy of unpreserved stools. D. fragilis was seen mainly in patients aged 0–30 and was not associated with travel activity less than 3 months prior to the submission of stools, suggesting endemic occurrence in Denmark.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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