Salmonella enterica serotype Typhimurium DT104 as a cause of infantile bacteraemia in Southern Mozambique

Abstract number: 1733_11

Ruiz J., Mandomando I., Macete E., Puyol L., Echeita A., Alonso P.

Objectives: To report the presence of Salmonella enterica serotype Typhimurium DT104 as a cause of infantile bacteraemia in Southern Mozambique.

Methods: Bacterial infections surveillance has been carried out at Manhiça Health Research Centre since 1998, Maputo, Mozambique. One hundred seventy Salmonella typhimurium isolated from under 15 children admitted at Manhiça District Hospital (MDH) were analysed. Microbial identification was performed by biochemical methods, while serotype and phagotype were established following conventional methodologies. Antimicrobial susceptibility levels to ampicillin (Amp), amoxicillin plus clavulanic acid (AMC), ceftazidime (Caz) gentamicin (Gm), streptomycin (Str) cotrimoxazole (Sxt), tetracycline (Tc), chloramphenicol (Chl), nalidixic acid (Nal) and ciprofloxacin (Cip) were established by the method of Kirby-Bauer. Molecular mechanisms of resistance to b-lactams, chloramphenicol and tetracycline as well as the presence of type 1 integrons were detected by PCR and sequencing.

Results: Two isolates were identified as S. typhimurium DT104, which were resistant to Amp, Str, Chl and Tc, being susceptible to Sxt, Caz, Nal, Cip and Gm. One of the isolates was susceptible to AMC, while the other one was intermediate. The presence of the genes carb2, floR and tetG, as well as two integrons, that carry on the aforementioned carb2 gene and an aadA2 respectively were detected. Presence of the tetA, tetB, cmlA, tem-like, shv-like, oxa-1 like, oxa-2 like and oxa-5 like genes was not detected.

Conclusion: This is the first report of multi-drug resistant Salmonella enterica serotype Typhimurium DT104, as responsible for bacteraemia in rural Mozambican children. Bacterial surveillance studies are need in the country to monitor the emergence of multi-drug resistant Salmonella including this phagotype.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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