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Non-tuberculous mycobacteria in a Spanish teaching hospital during a five-year period

Abstract number: 1732_290

Domingo D., Carracedo R., Fillol R., Alarcon T., Moreno M., Agudo S., Lopez-Brea M.

Objective: to know the prevalence of nontuberculous mycobacteria (NTM) isolated in a teaching hospital during a five-year period.

Methods: a total of 21,186 specimens obtained from patients attended at Area 2 of Madrid were studied. Samples were processed by standard methodology and were inoculated onto solid (Lowenstein and Coletsos) and liquid media using a semiautomatic equipment (MGIT®). Mycobacterium tuberculosis complex, M. avium complex, M. gordonae and M. kansasii strains were identified by DNA probes (Accuprobe®) and the rest were studied by biochemical tests, PCR-RFLP or sent to a Reference Laboratory.

Results: a total of 645 mycobacteria were isolated from 324 patients. M. tuberculosis complex was the mycobacteria most frequently isolated: 431/645 (66.8%). NTM isolates (336 strains from 111 patients) are summarised in the table.

Non-tuberculous mycobacteria isolated

MycobacteriaIsolatesPatients
M. avium complex1225
M. fortuitum1614
M. gordonae1511
M. kansasii145
M. abscessus143
M. simiae1614
M. chelonae76
Others*1312
Others: M. lentiflavum (3), M. mucogenicum (2), M. septicum (1), M. thermoresistibile (3), M. xenopi (3) and M. porcinum (1). A small percentage of NTM (3.1%) was isolated from non-respiratory samples. In 35 patients (31.5%), NTM were isolated in at least two specimens.

Conclusions:

1. NTM involve an important burden in the clinical mycrobiology laboratory.

2. Mycobacterium avium complex is the NTM most frequent in our study.

3. Although the isolation of NTM from repeated specimens of the same patient is not rare (31.5%), the clinical relevance of these data remains controversial.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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