Preliminary study on colonisation of A. baumannii in paediatric intensive care unit
Abstract number: r2138
Guducuoglu H., Aygul K., Yaman G., Izci H., Berktas M.
In recent months, there have been several outbreaks in various Pediatric Intensive Care Units (PICU) in our country with high mortality rates. For this reason, we planned to investigate the possible colonisations in PICU of our hospital and evaluate the clonal relatedness of the clinical samples. And we also planned to eradicate the pathogen microorganisms from this unit to prevent an outbreak.
For this reason many samples were collected from PICU of our hospital and then the isolated microorganisms were identified by PhoenixTM NMIC/ID, Becton Dickinson, USA kits. The results were also confirmed by conventional methods.
As a result, a total of 23 microorganisms were isolated from the samples of the screening. Twenty of them were A. baumannii and A. baumannii/calcoaceticus complex, three of them were Escherichia coli, Enterobacter cloacae and Acinetobacter lwoffii/haemolyticus. A. baumannii and A.baumannii/calcoaceticus complex strains were isolated from different samples like ventilation device, entubation sets, medicine pumps of some patients, naked hand of mothers and staffs, and head, neck and abdominal sites of some patients. These microorganisms were evaluated as colonisation. The antimicrobial resistance rates of A. baumannii and A. baumannii/calcoaceticus complex strains were as followed: amikacin 60%, aztreonam 100%, cefepime 100%, ceftazidime 100%, chloramphenicol 100%, ciprofloxacin 15%, gentamicin 100%, imipenem 5%, levofloxacin 15%, meropenem 10%, piperacillin 100%, trimethoprim/sulphamethoxazole 20%.
As a result of this study, we detected a colonisation of A. baumannii and A. baumannii/calcoaceticus complex in our PICU and it was found out that these microorganisms have gained high resistance to multiple antimicrobial agents. The clonal relatedness and the source of origin of these strains will be investigated by pulsed field gel electrophoresis. Meanwhile if any new Acinetobacter strains will be isolated from clinical samples from PICU of our hospital, the new isolates will be compared and the clonal relatedness will be investigated with the colonized bacteria. We gave advices for the eradication of Acinetobacter, education of the employees and taking serious preventions on controlling colonisations.
|Session name:||XXIst ISTH Congress|
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