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Diagnosis of Toxoplasmagondii by polymerase chain reaction method in pregnant women from Turkey

Abstract number: r2057

Dinc  B., Bozdayi  G., Biri  A., Kustimur  S.

Objective: 

To evaluate the prevalance of toxoplasmosis in various samples sent from Obstetrics and Gynaecology clinic to Gazi University molecular diagnosis laboratory by PCR.

Methods: 

One hundred and eighteen samples (90 blood, 27 amniotic fluid, one cord blood) obtained from 109 pregnant women during the 20th week of pregnancy for genetic diagnosis were enrolled in the study. Toxoplasma gondii DNA was extracted by Heliosis™ DNA Extraction kit (Metis Biotechnology, Turkey). Amplification was performed in two steps by using specific primers for the Toxoplasma gondii B1 gene. The second PCR products were analysed on a 1.5% agarose gel stained with ethidium bromide, and the expected 190 base pair length was confirmed.

Conclusion: 

PCR method can be used in prenatal diagnosis of congenital toxoplasmosis and acute infections in immunosuppressed patients by using various clinical samples such as blood, cerebrospinal fluid, amniotic fluid, bronchoalveoler lavage and urine especially in patients with atypical clinical and serological findings in the diagnosis of toxoplasmosis. In conclusion, we recommend to use PCR, as a direct and rapid assay to support the serological assays in pregnant women.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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