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Detection by PCR method of fastidious micro-organisms in urine from patients with UTI symptoms

Abstract number: r2044

Voiko  R., Remmet  J.

Objectives: 

We aimed to demonstrate to clinicians the advantage of detection by PCR of the fastidious microorganisms in urine from the patients with UTI symptoms, when standard cultures were negative and antimicrobial drugs were not applied to patients.

Methods: 

During the period of 08–12, 2004, 1326 urine samples were sent to the laboratory of microbiology from the nephrological department of the hospital. In standard cultures onto the CLED agar and blood agar the samples from 662 patients (49%) showed no growth. From among these patients, 113 were selected for further investigation with PCR method (within age range of 17–47 years, not cured with antibiotics). Urine samples of these patients were investigated with Roche Cobas Amplicor for Chlamydiatrachomatis and Neisseria gonorrhoeae, and with in-house PCR for Ureaplasma urealyticum, Mycoplasmahominis and Trichomonas vaginalis. The patients were divided into 3 age groups. Out of 113 patients 71% were women, what witnesses once again that UTI is prevalent among women due to anatomical peculiarities of their urogenital tract.

Results: 

From 113 samples investigated, 42 (37%) gave positive results with PCR methods, with microorganisms detected as follows: C. trachomatis– in 4% of samples, U. urealyticum– 29%, M. hominis– 12%. N. gonorrhoeae and T. vaginalis were not found. The age group of under 25 years, containing 36 samples, gave the following results: C. trachomatis– in 11% of samples of the respective group, U. urealyticum– 28%, M. hominis– 11%. In the age group of 26-35 years (44 samples), C. trachomatis was not found, U. urealyticum– 27%, M. hominis– 14%. In the age group of over 35 years (33 samples), C. trachomatis was not found either, U. urealyticum– 33%, M. hominis– 12%. In samples from women (80 samples), microorganisms were detected as follows: C. trachomatis– 11%, U. urealyticum– 31%, M. hominis– 11%. Among men (33 samples), percent of microorganisms found was: C. trachomatis– 9%, U. urealyticum– 24%, M. hominis– 15%.

Conclusion: 

In the case of negative results of standard urine cultures, investigation with PCR methods for detection of fastidious micro-organisms is of great help to clinicians. But it should be considered that 60% of healthy women carry U. urealyticum and 20% carry M. hominis in their urogenital tract. Unlikely, in male urogenital tract these microorganisms should not appear. If present in male urethra, they should always be treated as pathogens.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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