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Genotyping methicillin-resistant Staphylococcus aureus by PFGE in a clinical hospital in Szczecin, Poland: 9-year study

Abstract number: r1999

Kopron  K., Jursa-Kulesza  J., Giedrys-Kalemba  S.

Objective: 

The purpose of the study was genetic relatedness between MRSA strains isolated from different patients hospitalized in various wards of Clinical Hospital.

Material and method: 

A total of 270 MRSA isolates were cultured between 1994–2004 from various materials (blood, bronchoalveolar washings, sputum, secretion from wounds, catheters, drains, liquids from pleura and peritoneum) from patients hospitalized in various wards of Clinical Hospital in Szczecin: intensive care unit, surgery, internal medicine, urology, dermatology, cardiology. PFGE – SmaI was applied to analyze genetic relatedness among the isolates. Seperation of DNA fragments was achived using a CHEF DR II apparatus (Bio-Rad). The obtained patterns were compared by software Molecular Analyst Fingerprinting Software, using the Dice coefficient.

Results: 

Among the 264 isolates macrorestriction with SmaI and PFGE yielded 13 clonal epidemic strains, designated as A-M, and 6 strains were identified by unique PFGE patterns. During the period 1994–1997 two main PFGE types were detected: type A (48 strains) - isolated in all hospitals wards and type B (6 strains) – isolated in cardiology and urology ward only. The appearance of new genetic types were observed in the wards of Clinical Hospital from the beginning 1998 to 2004. The most frequently types were: type D included 86 strains and type E – 25 strains. Both clonal strains (D, E) were isolated for 3–4 years in surgery ward and intensive care unit. Types G, H, I, K were found in all hospital wards and the occurrence of types C, F, J, L was typical for one ward.

Conclusion: 

Molecular diversity of MRSA strains isolated from various wards for 9 years shows on their exchange and probably different sources of MRSA strains in hospital ecosystem. On the other hand, isolation of the same genetic types from different wards and patients proves horizontal spreading of the MRSA clones in Clinical Hospital. Maintenance of clonal strains MRSA for months or years in hospital environmental needs more effective of MRSA hospital control.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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