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Nosocomial strain of Serratiamarcescens producing new type of extended-spectrum beta-lactamase transfers the multidrug-resistance

Abstract number: r1935

Babalova  M., Blahova  J., Kralikova  K., Krcmery  V.,  Sr.

Objectives: 

To monitor the production of extended-spectrum beta-lactamases and transferability of antibiotic resistance from clinical isolates of Serratiamarcescens.

Methods: 

The strain No. 37/364 was isolated from the sputum of a patient hospitalized in the Dept. of Anesthesiology of the Regional Hospital Pribram, Czech Republic. Antimicrobial susceptibility testing was performed by the disc diffusion test. ESBLs profile of strain was performed using double-disc diffusion test. Transfer of genes for antibiotic resistance was performed in mixed liquid cultures. As recipient strains were used Escherichiacoli K-12 No. 3110 rif+ and Proteusmirabilis P-38 rif+.

Results: 

The double disc diffusion test demonstrated an unusual broad spectrum of the activity of ESBLs hydrolyzing cephalosporins of higher generations (cefotaxime, ceftazidime, cefepime, cefoperazone) and aztreonam. The multidrug resistant strain of Serratiamarcescens transferred directly to E. coli K-12 No. 3110 rif+ determinants of resistance to kanamycin, ticarcillin, cephalosporins of all generations (cephalothin, cefotaxime, ceftazidime, cefoperazon) as well as to aztreonam. Indirect selection procedure confirmed co-transfer of total spectrum of antibiotic resistance: kanamycin, ticarcillin, cephalosporins of all generations and aztreonam.

Conclusion: 

Production of ESBL(s) hydrolyzing cephalosporins of higher generations and transferability of resistance showed the possibility of spreading of antibiotic resistance in clinically important strains in hospital environment.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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