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The study of cellular and tissue mechanisms of homeostasis of the system "helminth – host" during muscular trichinellosis and the phytoorigin antihelminthic effect

Abstract number: r1888

Zanginyan  H., Asatryan  A., Ghazaryan  L., Melkonyan  L., Mkrtchyan  R.

Objectives: 

The purpose of our work was the study of the influence of phytoantihelminthic on the structure and functional activity of tissues of larva of trichinella and on the structure and micromorphofunctional condition of muscles and barrier-organs of their hosts during mixed invasion by larvas of T. spiralis and T. pseudospiralis.

Methods: 

The experiment was undertaken to test rats of Wistar line.The animals were infected with per.os. of T. spiralis and T. pseudospiralis larvas. The infection dose was 10 larvas of each species for 1 gram body weight of rat.The following medicinal herbs were used as phytoantihelminthics in the ratio of 2:2:1–Plantago major L., Satureja Montana L. and Ranunculus acris L. Animals received the substance in food in a dose of 50 mg/kg body weight. The animals were lanced on 35th day after being infected.

Results: 

Since 16-th day after infection destruction of the larvas of the trichinell of both species and destruction of capsules round the larvas of the T. spiralis were observed among the infected animals. The intracapsular sarcoplasm completely changed into coarse-grained mass. In most of larvas of T. spiralis and T. pseudospiralis the tincterial properties of cuticle broke and the latter became hard-knobbed and teared away from the hypoderm here and there. Metachromasia of reproductive glands and of digestive system was observed. On specimen the dead larvas of T. pseudospiralis revealed. In muscles, in livers and intestines of rats the factors of pathogenesis were weakening and the characteristic lines of the compensatory and the regenerative processes were observed. In muscular tissue the connective-tissue cicatrixes were formated, sudstituting the destruction of the muscular fibres and their fragments. In the liver partial recovery of structure and architectonics of trabecules were observed. In the result of phytotherapy as a whole the infiltration of parenchyma of liver by leucocytal elements descended. In muscles, in livers and in intestines of rats as a whole the synthesis of RNA and formation of glycogen noticeably became intensive.

Conclusion: 

The phytoantihelminthic detained encapsulation of larvas of T.spiralis caused death of particular number of larvas of trichinella of both species. This work is of interest for the development and for application of plant origin antihelminthic during the therapy of helminthosies especially trichinellosis.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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