Detection of enterovirus, parvovirus B19 and human herpes virus type 6 by real-time PCR in blood samples of infants with febrile syndrome
Abstract number: p1881
García-Álvarez M., Folgueira M.D., Prieto C., Babiano M.J., Rojo P., Otero J.R.
Enterovirus, parvovirus B19 and human herpes virus type 6 (HHV-6) are a common cause of infection in young infants. The objective of this study was to determine what portion of the infants who received a clinical diagnosis of febrile syndrome have a viral etiology by these three genera of viruses.
Ninety-six patients were included in the study, all of them were admitted to the pediatric casualty of a tertiary care hospital, and all of them presented a febrile syndrome without a clear focus of infection (urinary tract, lung and meningeal infections were discarded). The assay was carried out in 96 blood samples by real-time PCR. DNA was isolated from 200 ml of blood by semi-automated system MagNA Pure LC Total Nucleic Acid Isolation kit (Roche Diagnostics, Nederland BV). PCR was performed in a LightCycler instrument (Roche Molecular Biochemicals) by a uniform cycling parameters: 10 min at 95°C for polymerase activation, and 50 cycles of 15 s at 95°C and 60 s at 60°C for amplification of the specific target sequence (5 UTR gene for enterovirus, VP2 gene for parvovirus B19 and DNA polymerase gene for HHV-6). PCR product formation was detected continuously by the use of TaqMan probes.
A viral amplification was detected in 52 (54%) of the 96 patients included in this study. Enterovirus was detected in 12 (12.5%) of the patients, parvovirus B19 in 10 (10.4%) and HHV-6 in 35 (36.4%). In five cases two viral amplifications were detected at the same time: 3 parvovirus B19/HHV-6 and 2 enterovirus/HHV-6. The mean age of the patients was 2 years old (range from 24 days to 14 years). In group of infants <6 months old (n = 27) there were 7 enterovirus and 6 HHV-6. In the infants from 7 months to 3 years old (n = 53) there were 3 enterovirus, 3 parvovirus and 24 HHV-6. In the last group of infants >3 years old (n = 16) there were 2 enterovirus and 7 parvovirus B19.
Viral infections are an important cause of sepsis in infants admitted to hospital. Enterovirus was the most frequent virus detected in infants <6 months, parvovirus B19 the most frequent in children >3 years old, and the HHV-6 was detected in all age groups. Qualitative real-time PCR in blood is a rapid and sensitive method for diagnosis of enterovirus and parvovirus. However, is not the better method for diagnosis of HHV-6, a latent virus, in which this technique is not capable of distinguish between recent and acute infection.
|Session name:||XXIst ISTH Congress|
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