Room disinfection in the hospital setting using Akacid plus®

Abstract number: p1792

Kratzer  C., Tobudic  S., Graninger  W., Buxbaum  A., Georgopoulos  A.


Akacid plus®, a novel polymeric guanidine with broad antimicrobial activity also against multi-resistant bacterial strains, was used in the present study as room disinfectant.


Disinfection of closed rooms experimentally contaminated with antibiotic-susceptible and multi-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Escherichia coli was performed using Akacid plus® at concentrations of 0.1%, 0.25% and 0.5% for 100 minutes. Bacterial suspensions were distributed on stainless steel plates and placed in a test and control room. Recovery of the test bacteria was determined

before nebulizing, 60 and 100 minutes after the beginning and 4 hours after the end of room disinfection by a modified simple swab-rinse technique. For the detection of MRSA in isolation units, surface samples were collected by direct swab and enrichment culture.


The swab-rinse method demonstrated a dose- and time-dependent effectiveness of Akacid plus® in eradicating S. aureus, E. coli and P. aeruginosa on stainless steel plates. Nebulizing of 0.5% Akacid plus was successful in eliminating all hospital pathogens in 340 min contact time, while MRSA was still detectable after use of 0.25% Akacid plus®. 0.1% Akacid plus® achieved a reduction >100 CFU of S. aureus and P. aeruginosa, but was only able to eradicate E. coli during the observation time.


The results suggest that nebulized Akacid plus® at a concentration of 0.5% is a potent substance for eradication of pathogenic organisms in the hospital setting.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Location: Oxford, UK
Presentation type:
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