Direct detection of Cardiobacterium hominis by broad-range 16S rRNA PCR and sequencing in the serum of a patient with infective endocarditis
Abstract number: p1759
Malli E., Klapsa D., Vasdeki A., Morava M., Pitsitaki M., Petinaki E., Maniatis A.
To describe the detection of Cardiobacterium hominis directly in the serum of a patient with infective endocarditis, by employment of broad-range 16S rRNA PCR followed by sequencing.
A series of blood cultures were taken from the patient before starting empirical treatment. In addition, 10 ml whole blood was collected in rubber sealed pyrogen-free tubes for direct detection of bacterial DNA. Bacterial DNA was detected by a broad range PCR reaction and sequencing process allowed identification of bacteria species.
Cardiobacterium hominis was identified as the causative agent of infective endocarditis, on two days after the serum collection. Blood cultures, simultaneously obtained with the serum sample, remained negative after 5 days of routine incubation; however, after a prolonged incubation of twelve days a gram negative bacterium was isolated from the aerobic bottles, that was identified as C. hominis species, by the usual phenotypic studies (catalase, oxidase reaction, indole, nitrate, etc) which are time-consuming.
To our knowledge this is the first report of direct detection of C. hominis in the serum using molecular methods, emphasizing the need for the establishment of such methods especially for infections caused by fastidious organisms.
|Session name:||XXIst ISTH Congress|
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