Detection of calicivirus genome in calves using Ni/E3 primers

Abstract number: p1673

Mahzounieh  M., Zahraeisalehi  T., Moghtadaei Khorasgani  E.

Caliciviruses may cause a wide spectrum of disease in animals and are important etiological agent of viral gastroenteritis in humans. Members of the family caliciviridae are small nonenveloped viruses 27 to 35 nm in diameter. They possess a single stranded poly adenylated RNA genome. Caliciviruses have been isolated from mink, dog, cattle and non-human primates. "Norwalk-like viruses" (NLVs) are the most common cause of acute non-bacterial gastroenteritis in humans. Cattle may be a reservoir of NLVs although never bovine NLVs have been found in humans. In this study, we try to detect enteric caliciviruses genome from 50 faecal samples of 6 dairy cattle herds in Shahrekord area using Reverse transcriptase polymerase chain reaction (RT-PCR) assays specific for NLVs found in humans. The primers used for PCR amplification were Ni and E3, which amplify a 113-bp product for the detection of both genogroups I and II SRSV RNA in fecal material. Our results showed that nine specimens (18%) were positive. These findings suggest that calicivirus infection is endemic in dairy herds in Shahrekord, Iran and may be have an important role in calf diarrhea.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Location: Oxford, UK
Presentation type:
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