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Genetic diversity and molecular epidemiology of respiratory syncytial virus over six consecutive seasons in Cuba

Abstract number: p1658

Valdes  O., Savon  C., Goyenechea  A., Palerm  L., Gonzalez  Gr., Gonzalez  Gu., Melero  J., Martinez  I.

Respiratory Syncytial Virus (RSV) is a major cause of acute lower respiratory tract infection in infants and young children. It has previously been shown that HRSV isolates can be divided into two antigens groups A and B. The G protein is the most divergent both between and within the two subgroups and appears to accumulate amino acid changes with time, suggesting evolution under selective pressure. Our knowledge of the molecular epidemiology of RSV has so far been based mainly on studies done in the developed world with e temperate climate. Very limited epidemiological data are available from tropical and developing countries, where RSV infections may follow a different pattern. In this report we examine the molecular epidemiology and evolutionary pattern of the G protein of both subgroups A and B RSV through consecutive epidemics in Cuba. Sixthly four nasopharyngeal swabs were collected from children under 1 years of age with respiratory disease to different hospitals in Cuba between 1994 and 2000, to examine the molecular epidemiology and evolutionary pattern of the G protein of RSV. All samples collected from 1994 to 1999 were RSV subgroup A; however both subgroups co-circulate during 2000. The Cuban isolated from 1994 to 1996 showed a great homogeneity between them and were resemble to an ancient strain (Long) with only five nucleotide differences, this also occur in 1998 and 2000 with two strain. Furthermore was detected different size of G protein (297 or 298 for RSV A and 295 for RSV B) due to change in stop codon used he genetic homogeneity of the Cuban isolates (1994–1996) and their resemble to an ancient strain such as Long was an unusual finding in our country. In both subgroups was observed the predominance of strains with almost similar sequences. Phylogenetic analysis for subgroup A strains showed that strains were cluster in different genotypes with virus isolated in different geographic regions. Both subgroups co-circulated during 2000 and clustered whit South African strains that circulate during at the same time.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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