Detection of plasmid-mediated low-level resistance to fluoroquinolones in Enterobacter cloacae
Abstract number: p1250
Higgins P., Seifert H., Wisplinghoff H.
Low-level resistance to fluoroquinolones encoded by the qnr gene and mediated by plasmid pMG252 has recently been observed in clinical Enterobacteriaceae isolates, mainly E. coli and K. pneumoniae.
To determine the prevalence of pMG252 carrying the qnr, 110 clinical isolates from hospitals and private practices in the Cologne Metropolitan area were screened using a PCR based assay.
Among 58 isolates from hospitalized patients with urinary tract infection (UTI) and 52 isolates from patients with community-acquired UTI, pMG252 was detected in one Enterobacter cloacae isolate from a hospitalized patient. The isolate displayed an intermediate susceptibility to ciprofloxacin (MIC, 2 mg/mL) and was susceptible to levoloxacin. Presence of pMG252 and the qnr gene was confirmed by DNA sequencing.
This is one of the first reports of pMG252 in a clinical E. cloacae isolate. Even though the prevalence of plasmid mediated resistance to fluoroquinolones in clinical isolates is low, pMG252 carrying the qnr gene has now been reported in most clinically relevant Enterobacteriaceae. Further studies monitoring the potential emergence of pMG252 positive strains may be warranted.
|Session name:||XXIst ISTH Congress|
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