Diagnosis of tuberculosis in suspects in endemic area by using enzyme-linked immunospot assay for gamma-interferon

Abstract number: p1102

Hsueh  P.R., Wang  J.Y., Chou  C.H., Lee  L.N., Hsu  H.L., Jan  I.S., Yang  P.C., Luh  K.T.


We evaluate the value of the ex vivo enzyme-linked immunospot assay for gamma interferon (TB-ELISPOT) for rapid diagnosis of active tuberculosis in clinical suspects in endemic area.


From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with tuberculosis were recruited and a blood sample was obtained for TB-ELISPOT assay within 7 days after the microbiologic studies were performed.


Sixty-five suspects were studied, including 39 (60%) with active tuberculosis. Thirty-five (53.8%) patients had underlying co-morbid conditions. Thirty-seven patients had positive cultures for M. tuberculosis, whereas nontuberculous mycobacteria were isolated in 11 patients. The overall sensitivity and specificity of the TB-ELISPOT assay were 87.2% and 88.5%, respectively. The positive and negative predictive values were 91.9% and 82.1%, respectively.


Our results showed that the accuracy of the TB-ELISPOT assay for active tuberculosis in clinical suspects is higher than 80%, even in an environment highly contaminated with nontuberculous mycobacteria.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Location: Oxford, UK
Presentation type:
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