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Diagnosis of tuberculosis in suspects in endemic area by using enzyme-linked immunospot assay for gamma-interferon

Abstract number: p1102

Hsueh  P.R., Wang  J.Y., Chou  C.H., Lee  L.N., Hsu  H.L., Jan  I.S., Yang  P.C., Luh  K.T.

Objectives: 

We evaluate the value of the ex vivo enzyme-linked immunospot assay for gamma interferon (TB-ELISPOT) for rapid diagnosis of active tuberculosis in clinical suspects in endemic area.

Methods: 

From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with tuberculosis were recruited and a blood sample was obtained for TB-ELISPOT assay within 7 days after the microbiologic studies were performed.

Results: 

Sixty-five suspects were studied, including 39 (60%) with active tuberculosis. Thirty-five (53.8%) patients had underlying co-morbid conditions. Thirty-seven patients had positive cultures for M. tuberculosis, whereas nontuberculous mycobacteria were isolated in 11 patients. The overall sensitivity and specificity of the TB-ELISPOT assay were 87.2% and 88.5%, respectively. The positive and negative predictive values were 91.9% and 82.1%, respectively.

Conclusion: 

Our results showed that the accuracy of the TB-ELISPOT assay for active tuberculosis in clinical suspects is higher than 80%, even in an environment highly contaminated with nontuberculous mycobacteria.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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