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Direct detection of Salmonella spp. in faecal specimens by real-time PCR assay

Abstract number: p941

Pachiadakis  I., Karavassilis  V., Vasdeki  A., Melidou  A., Goudouva  A., Lisgara  P., Petinaki  E., Maniatis  A.

Objective: 

To evaluate a commercial kit for the detection of Salmonella spp. in faecal specimens based on Real-Time PCR (Real-Art TM Salmonella RG PCR Kit).

Methods: 

A total of 1000 faecal specimens obtained consecutively from individual patients of Thessalia (Central Greece) were included in this study. Genomic DNA extractions were performed by use of commercially available isolation kits, while the commercial kit described above, according to the instructions of the manufacturer did real-time PCR. Results obtained from Real-Time PCR assay were compared with bacterial culture results to determine sensitivity and specificity.

Results: 

According to the results obtained by Real-Time PCR, fifty-three of 1000 faecal specimens were positive for Salmonella spp. Salmonella was detected by bacterial cultures in all, except two, the positive specimens. Repetition of two negative cultures after enrichment detected the pathogenic micro organisms. The mean time for the detection of Salmonella by Real-Time PCR was approximately 5–6 hours, while detection by traditional culture methods required 2–3 days.

Conclusion: 

The high sensitivity and specificity of this Real-Time PCR assay emphasizes the need of its application in a routine clinical laboratory for the rapid and accurate detection of Salmonella in faecal specimens.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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