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Nasal carriage of Staphylococcus aureus among patients and personnel of a haemodialysis unit

Abstract number: p885

Sergounioti  A., Sergouniotis  F., Basdeki  A., Sergouniotis  P., Papoulia   E., Petinaki  E.

Nasal carriage of Staphylococcus aureus portends an increased risk of peritonitis and other serious infections for haemodialysis patients, thus increasing morbidity and mortality.

Objectives: 

The aim of our study was to determine the incidence of S. aureus nasal carriage among patients and personnel of the Haemodialysis Unit of a Secondary General Hospital (120 beds) and to investigate the study of the genotypical and phenotypical characters of the strains yielded.

Methods: 

A total of 35 individuals were examined during autumn 2005. Samples taken from the anterior snares of the twenty-five haemodialysed patients and the ten personnel of the Haemodialysis Unit of our Hospital were cultured. The strains' identification was performed by catalase production, 24-hour coagulase test, and the API Staph system (Biomerieux, Marcy L'Étoile, France). All the strains were tested for the production of PBP2a (Slidex MRSA, Biomerieux, Marcy L'Étoile, France). Their susceptibility to antibiotics was performed by the disk diffusion technique and the results were interpreted according to the criteria of the N.C.C.L.S. Moreover, molecular analysis for the mecA gene and the Panton-Valentine leucocidin (PVL)-encoding genes, lukF and lukS, was performed by PCR.

Results: 

The prevalence of S. aureus nasal carriage among patients was 48% (12 out of 25) and 20% among personnel (2 out of 10). The strains isolated from the patients were all MSSA and did not carry the PVL-encoding genes. All the MSSA strains were susceptible to glycopeptides, linezolid, quinopristin/dalfopristin, cotrimoxazole, quinolones, rifampicin, amikacin, gentamicin, and netilmicin, whereas their susceptibility to fucidic acid, erythromycin and clindamycin was 92%, to tetracycline 85% and to penicillin 23% respectively. Only one strain carrying the mecA gene was recovered. This MRSA strain was isolated from a nurse and proved to be multisusceptible.

Conclusions: 

The haemodialysis unit of our hospital was found to have higher incidence of S. aureus nasal carriage among patients and personnel compared to other larger haemodialysis units of tertiary hospitals in Greece. However, MRSA carriage was rare and the most of the S. aureus strains isolated were multisusceptible.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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