Diagnosis of Giardia lamblia with microscopy, striptest, ELISA and real-time PCR
Abstract number: p856
Brinkman M., Vastert D., Wilke H., Mulder B.
Giardia lamblia is the most frequently diagnosed pathogenic intestinal parasite in the Netherlands. We compared four different diagnostic methods for the detection of G. lamblia in faeces in both acute and chronic diarrhoea.
Microscopic examination was carried out on stained samples collected with triple faeces test (TFT). ELISA (Novitec and Novatec Giardia lamblia ELISA), Giardia-strip (Coris Bioconcept), and real time PCR for the detection of G. lamblia were performed on corresponding fresh stool samples.
Five hundred and fifteen faeces were included. 154 fresh watery specimens from acute diarrhoea were sent for bacteriological examination and 361 TFT-samples, representing a more chronic form of diarrhoea, were sent to the parasitology department. Using real time PCR as the gold standard, the positive predictive values of microscopy, ELISA and Giardia-strip were 100%, 99% and 50%, respectively. The sensitivity of microscopic detection was 71% while that for Giardia-strip was only 5%. Novitec ELISA was more sensitive (67%) than Novatec ELISA (51%). Specificity of all methods was never lower than 97%.
Microscopy with triple faeces test is a very specific method for the laboratory detection of G. lamblia in faeces with good sensitivity. Both ELISA's also have good sensitivity and can be used as acceptable alternatives for microscopy. Giardia-strip can not be used as an alternative, because of its very low sensitivity. Real time PCR is a very sensitive and specific method for the detection of G. lamblia.
|Session name:||XXIst ISTH Congress|
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