The effectiviness of impregnation of graft with cefazolin in foreign body infection
Abstract number: p839
Kilic D., Agalar C., Denkbas E., Agalar F., Ozturk E., Emirdogan M., Deveci O.
Foreign body infection (FBI) is a real problem in clinical situations. In the present study, in vitro and in vivo efficiacy of impraganation of mesh with cefazolin in prevention of FBI.
Materials and methods:
Strain: The microorganism was slime positive, methicillin-resistant S. epidermidis (MRSE). Impregnation: Cefazolin impregnated grafts were prepared by dipping method. Naive meshes, in size of 5 × 10 mm were immersed into the polylactic acid (PLA) solution in dichlorometahane including cefazolin with different concentrations (i.e., 0.025, 0.05 and 0.3 g sefazolin/5 ml and 2% w/v of PLA solution). Cefazolin impregnated and naive grafts with different concentration (Group 1 = 0.025 g, Group 2 = 0.05 g, and Group 3 = 0.3 g) were incubated with 108 cfu/ml slime positive MRSE. In vitro: After 24 and 48 hours of incubation, the numbers of colonies were counted in an aliquot and adhered to catheter. In vivo: Contaminated naïve and cefazolin-impregnated grafts (n = 10 in each groups) were implanted subcutaneously in the back of Swiss albino mouse. Grafts were explanted at 7 days following implantation. Microbiologic assessments and electron microscopic (JOEL JSM-5600 Japan) evaluation of catheter segments were performed.
Impregnation of cefazolin decreased the numbers of adherent bacteria to the grafts and the number of free bacteria within the liquid medium significantly in all groups. In all comparisons; the decrease of bacterial counts were statistically significant, except the values at 48 hours of group 2 vs group 3. Cfu counts in explanted grafts were significantly less in treatment than in control group and wound infection rates also decreased, accordingly.
Impregnation of cefazolin to grafts by dipping method may protect against FBI with MRSE during peri-operative period.
|Session name:||XXIst ISTH Congress|
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