Detection of herpes simplex virus in cerebrospinal fluid using real-time PCR
Abstract number: p750
To evaluate the performance of the Light Cycler HSV 1/2 Detection Kit (Roche) in 124 cerebrospinal fluid (CSF) samples from adult patients.
124 CSF samples from adult patients admitted to our hospital since May 2004 to September 2005 were studied. Thirty-five of them showed a typical glucose, protein and cell count pattern of viral infection. After specific cultures, 100 ml were separated and frozen ((80°C) until further study. We performed nucleic acid extraction with the High Pure Viral Nucleic Acid Kit (Roche) following the manufacturer's recommendations. An internal control was included in each sample. The Light Cycler HSV 1/2 also includes a positive control which, after amplification shows two melting temperature picks; 54°C and 66.5°C (corresponding to HSV-1 and HSV-2 respectively). We also analysed four cell cultures infected with HSV (two with HSV-1 and two with HSV-2) to assess the whole process of extraction and real time PCR.
One of the samples yielded a positive result to HSV-2. It's biochemical characteristics and the clinical pattern of the patient were compatible with a viral meningoencephalitis. The four infected cultures were also positive. The rest of the samples yielded negative results but all of them showed amplification of the internal control. The whole process (extraction and amplification-detection) took approximately 2 hours.
(1) The presence of HSV in CSF samples in our hospital is rare. (2) The HSV 1/2 Detection Kit allows rapid detection and differentiation of HSV-1 and HSV-2 in CSF samples and so can be a helpful tool in management of central nervous system infections.
|Session name:||XXIst ISTH Congress|
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