Detection of human papilloma virus (Type 16, 18) in pathological sample from patients with cervical cancer by PCR and RFLP methods
Abstract number: p639
Maleknejad P., Shahsavan S.
Infection with human papilloma virus (HPV is the most frequent sexually transmitted disease world wide. HPV types 16, 18, 31 and 33 are considered as important causes of cervical cancer. This study was carried out to detect HPV types 16 and 18 among 64 pathologic blocks from patients with cervical cancer in Tehran.
Primers HP6133 F 5'-TGG ATT ATA AAC AAA CAC A-3', HP6133 R 5'-GTG GTA TCT ACC ACA GTA ACA-3', HP168 F for types 6, 11, 31 and 33 and 5'-GAA TAT GAT TTR CAG TTT ATT TT-3', HP168 R 5'-TCT YKA GAA AAC TTT TCC TTT-3' for types 16, 18 and 35 were designed from the sequences of HPV (Genebank accession numbers E54157, U34171, M74117, AF067049) and used in PCR. These primers produced amplicons with sizes of 564 and 269 bp respectively. The PCR products were digested with BamHI and EcoRI and the fragments were separated by electrophoresis in agarose gel.
Human papilloma virus DNA was detected in (59.4%) of the cases. HPV type 16 was the most common one (22/64, 34%) followed by HPV type 18 (16/64, 25%). Digestion of PCR products with BamHI and EcoRI differentiated type 16 and 18 respectively as the amplicons from each type had one restriction site for one of the enzymes used.
HPV type 16 was the predominant infection (34%) in our study. This rate in our country is still lower than in other countries such as Croatia (50%), Australia (53%), Spain (66%) and China (48.8%). However, in compare with the previous study in Iran (26.7%) infection with type 16 shows an increase. Of 16 cases infected with HPV18, 11 had squamous cell carcinoma. Statistically, there was significant correlations between infection with HPV type 18 and development of squamous cell carcinoma (p = 0.019). Such relation was not found for type 16. PCR and PCR-RFLP are sensitive and useful in identification and typing of papiloma virus and differentiation of their types.
|Session name:||XXIst ISTH Congress|
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