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Detection of Coxiella burnetii and anti-Coxiella antibodies by molecular and serological methods among risk groups

Abstract number: p558

Eyigor  M., Kirkan  S., Gultekin  B., Yaman  S., Tekbiyik  S., Aydin  N.

Objectives: 

Q fever is due to Coxiella burnetii and usually found as a professional fever disease and can be presented in its acute or chronic forms. Isolation of C. burnetii should be done in a 3rd level security laboratory which increase the value of serologic diagnosis for the institutions which do not have it. The aim of this study was detection of C. burnetii and anti-Coxiella antibodies by molecular and serological methods among risk groups.

Methods: 

Of 92 people studied were 85 males and 7 females with ages ranging from 18 to 60 years. Among 92 people, 30 were veterinary doctors, 30 were farm workers and 32 were butchers. Sera were collected from all 92 people and presence of anti-Coxiella burnetii antibodies was studied using C. burnetii ELISA IgG and C. burnetii ELISA IgM kits (Vircell, Spain). The positive or equivocal samples with ELISA were studied further by IFA. IFA test were done using Coxiella burnetii Phase I+II kits (Vircell, Spain). Presence of C. burnetii was also studied in all cases by PCR using specific primers Trans1: 5'-TGGTATTCTTGCCGATGAC-3', Trans 2: 5'-GATCGTAACTGCTTAATAAACCG-3'.

Results: 

A total of 12 (13.0%) and 8 (8.7%) people were positive and equivocal by ELISA IgM, respectively. Among 92 people studied 32 (34.8 %) and 9 (9.8%) people were positive and equivocal by ELISA IgG, respectively. The ELISA positive and equivocal sera were studied further by IFA and in 7 (7.6%) cases IgM and in 39 (42.4%) cases IgG presence were confirmed. All IgM positive cases were also positive for IgG but one, so 40 (43.5%) cases were C. burnetii seropositive. There was no significant difference for C. burnetii seropositivity among three professional groups (veterinary doctors, farm workers and butchers) (p > 0.05). Only 4 (4.3%) cases PCR was positive.

Conclusions: 

Coxiella seropositivity was found to be 43.5% among risk groups which is higher than the rates reported among general population in Turkey. For this reason especially among risk groups in case of atypical pneumoniae, granulomatous hepatitis, and fever with unknown aetiology, Q fever should be thought and searched for differential diagnosis.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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