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Detection and identification of bacterial DNA in cardiac valves from infective endocarditis cases

Abstract number: p543

Kemp  M., Bangsborg  J., Kjærulf  A., Schmidt  T., Christensen  J., Irmukhamedov  A., Bruun  N.E., Dargis  R., Andresen  K., Christensen  J.J.

Objectives: 

The study investigates the usefulness of culture-independent PCR and subsequent DNA sequencing for establishing the aetiology of infective endocarditis (IE) in surgically removed valves.

Methods: 

Cardiac valves from patients undergoing valvular replacement due to clinical necessity were examined by culture and by PCR/DNA sequencing. Valves from ten patients without suspicion of IE (controls) and from13 patients with IE (patients) were included. The valves were divided and one part was cultured using standard methods, and the other was subjected to a PCR targeting part of the bacterial 16S rRNA gene. The DNA sequence was determined on any product resulting from PCR and bacterial identity was established using BLAST search in the NCBI database.

Results: 

Results from culture and PCR /DNA sequencing are shown in Table 1. Bacterial DNA was identified in valves from nine of the 13 patients. The species identified represented typical endocarditis bacteria in eight of these patients. Six of the DNA based identifications were in accordance with previous blood isolates from the same patients. DNA from Propionibacterium acnes was detected in two of the ten controls, and may represent contamination.

Conclusion: 

Detection and identification of bacterial DNA seems a promising method for establishment of the aetiology of IE, but further studies are required for final validation.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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