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Identification of genital mycoplasmas: comparison of two methods

Abstract number: 1135_163

Kouskouni E., Logginidis J., Salomidou P., Efstratiou V., Makrakis E., Hassiakos D., Baka S.

Objectives:  

Mycoplasmas represent a unique group of microorganisms. Mycoplasma hominis (Mh) and ureaplasma urealyticum (Uu) are common genital inhabitants that have been associated with a variety of clinical conditions in pregnancy, infertility and pelvic inflammatory disease. The aim of the study was to compare two methods for the isolation of the organisms from the lower genital tract.

Methods:  

We studied 97 vaginal secretions from an equal number of women attending our hospital. All samples were cultured for aerobic and anaerobic bacteria. For the identification of both Mh and Uu two methods were used. One was the commercial kit Mycoplasma IST 2 (BioMerieux, France) and the other was an ‘in house’ method which is used for years in our laboratory. For the identification of Mh we prepared DNA-PPLO agar, while Uu was identified by its ability to metabolize urea using T-broth, a preparation containing 10% urea broth.

Results:  

Out of the 97 samples, with the commercial kit we isolated 26 positive samples (26.8%) for Uu and 6 positive (6.2%) for Mh. On the other hand, our method resulted in 28 (28.9%) positive samples for Uu and 4 (4.1%) positive for Mh. The commercial kit offers the advantage of performing the antibiotic susceptibility testing. The most sensitive antibiotics for mycoplasmas were tetracycline (96.2%) and deoxycycline (92.2%). To our surprise, quinolones (Ofloxacin and Ciprofloxacin) showed decreased susceptibility rates (26.9% and 3.8%, respectively), while the intermediate sensitivity rates were very high (69.3% and 73.1%, respectively). In 42.3% of the cases Uu was the only pathogen isolated. Uu coexisted with bacterial vaginosis in 38.4% of cases, while with other pathogens like Gram-negative rods, Gram-positive cocci and yeasts in 11.5%, 3.9% and 3.9%, respectively. Concurrent infections by both Uu and Mh were in all cases accompanied by bacterial vaginosis.

Conclusions:  

The two methods proved to be similar in sensitivity and specificity. The commercial kit has the advantage of the antibiotic susceptibility testing. Mycoplasmas were moderately sensitive to quinolones probably because of the irrational use over the last years. Both Uu and Mh correlated positively with bacteria involved in the specific clinical entity bacterial vaginosis.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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