The antibiotic resistance and metallo-b-lactamase production of carbapenem-resistant pseudomonas and acinetobacter

Abstract number: 1135_33

Yildirim F., Sengoz G., Yasar K.K., Karabela S., Berzeg D., Kutlu S.B., Durdu Y.B., Nazlican O.


The betalactamase resistance mechanism are, outer membrane protein mutation, efflux pumbs and metallo-betalactamases (MBL). MBL enzymes which uses Zinc ion can hydrolyse betalactamase antibiotics and are usually found in Enterobactericeae ve and especially in Pseudomonas aeruginosa like nonfermentative bacteria. The genes encoding MBL are named as IMP, VIM, SPM and GIM, usually transmitted via plasmids. Their importance are that they hydrolyse all betalactam and betalactam inhibitor combinations except aztreonam. Especially the abundance of strains producing MBL in hospitals must be evaluated with care.


In our study carbapenem resistant 13 Pseudomonas aeruginosa and 10 Acinetobacter spp. strains isolated from various clinical samples sent to our clinical microbiology laboratory were tested for MBL production by MBL E test and for their susceptibilities for ciprofloxacin (CIP), gentamicin, amicasin, aztreonam, piperacillin-tazobactam (TZP), ve cephaperazon-sulbactam by disk diffusion test.


Out of Carbapenem resistant 23 strains 21 were found MBL positive with E Test method. 23 strains were sensitive 29% for TZP, 28% for CIP, 17% for gentamicin, 19% for amikacin and 18% for cephaperazon sulbactam. Carbapenem resistant strains were mostly resistant also for the other antibiotics.


As a result carbapenem resistance in Gram negative aerob and anaerobe bacteria due to MBL is a popular but difficult problem to solve.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Location: Oxford, UK
Presentation type:
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