Determination of drug resistance in Pseudomonasaeruginosa isolated from burn patients in Sari, Iran 2004

Abstract number: 1134_04_161

Ahanjan M., Vahedi M., Nasrollahie M.

Objectives:  

Pseudomonasaeruginosa is the main opportunist pathogen One of the features of this organism is resistance to most of the current antibiotics, as a result creates problems in tratmentent . This sudy is on the determination of MIC of currently used antibiotics to Pseudomonas .Therefore, it is necessary to have study on this phenomenon. We tried to have a regional on the drug resistance in the pseudomonas isolated in burn patients referring to burn treatment centre of zareh hospital ,in order to the patients be benefited from the more effective antibiotic treatment.

Methods:  

With the help of sterile swab, sample from the burn was collected, kept in tube containing sterile saline and transported to the laboratory .cultured on blood agar and EMBagar,incubated at 37°(D.C) centigrade for 24 hours. Gram staining was done from the.colonies belonging to the gram negative and oxidas positive bacilli were selected and cultured on simmon citrate and TSI, incubated at 37 D.C for 24 hours. The confirmed P.aeruginosa colonies were inoculated in sterile saline, suspension was prepared and compared with 0.5 standard of Mac Farland . Then cultured by streak method on Muller–Hinton. Antibiogram was done using disk of antibiotic prepared from Padten Teb company ,Iran. Kept at 37D°C for 24 hours. The resistance and sensitivity to different antibiotics were determined in millimeters comparing with the chart provided from the manufacture.

Results:  

In this study samples were collected from 250 patients admitted in burn unit of zareh hospital. 60% of the patients under study had Pseudomonas infection, There was significant difference between prevalence sex, Geographic condition hospital stay period. It was shown that resistance to Amikacin, Gentamycin, trimethoprim sulfo methoxazole, cephoxidim, ceftazidim. Ciprofloxazin, cephipenem, emipenem, and cephriaxon, 72.9% , 63% , 41.6% , 33.3% , 30% , 29.1% , 27% , 25% , and 15% respectively.

Conclusion:  

Result of the studies on resistance of the organisms to antibiotics differs in different countries. These are as follow: To Amikacin 11%( in spain ), 1% in Germany , 67% Tehran (Iran) and in this study 72.9%. Also resistance to Gentamicin 65.5% in zimbabveh, Tarbiat modarres university (Iran)78%, and in this study 63%. Considering the obtained results on the resistance of this organism to antibiotics, regional study on determination of drug resistance is necessary.