Ciprofloxacin impregnated carboxymethylcellulose membrane reduces adherence and intra-abdominal abscess formation and mortality rate in a lethal intra-abdominal sepsis model in mouse

Abstract number: 1134_04_137

Aydinuraz K., Agalar C., Saygun O., Denkbas E., Ozturk E., Cime A., Daphan C., Agalar F.

Objective:  

To assess the efficacy of ciprofloxacin impregnation to carboxymethylcellulose membrane in reduction of adherence, intraabdominal abscess formation and mortality rates in a lethal intraabdominal sepsis model in mouse.

Methods:  

A midline laparotomy was performed under general anesthesia and 0.8 ml of 3 × 10⁸ cfu/ml E. coli and 0.2 ml of sterile homogenated mouse faeces as an adjuvant were inoculated after caecal abrasion in mouse. This model was found to be 100% lethal in 24 hours. Seprafilm was dissolved in 0.05 M NaOH solution by sonication for 4 hours. Ciprofloxacin and 0.05 M HCl solution were added to this solution and mixed for 2 hours. Different drug concentrations were used in the preparation of membranes. At predecided intervals, samples were withdrawn and the release of ciprofloxacin in the buffer solution was followed by UV spectrophotometer at 275 nm. The cumulative drug released into the medium from membranes was measured as a function of time.Afterwards the study was conveyed in four groups. In the first group this newly synthetised carboxymethylcellulose membrane was placed after midline laparotomy and caecal abrasion without any microbiological intervention. Three ciprofloxacin impregnated carboxymethylcellulose sheets differing in ciprofloxacin doses were used in groups 2, 3 and 4. The mice were sacrificed on day 7 and the presence of intraabdominal abscesses and adhesions were evaluated

Results:  

The newly synthetised carboxymethylcellulose was found to be similar to the original with respect to intraabdominal adhesions. There was no mortality in groups 2, 3 and 4. The rate of intraabdominal abscess formation and adherence were significantly reduced in study groups 2, 3 and 4. The rate of intraabdominal adhesion and abscess formation were reduced as the amount of impregnated ciprofloxacin was increased.

Conclusion:  

The impregnation of ciprofloxacin to newly synthetised carboxymethylcellulose reduces mortality rate, intraabdominal abscess and adhesion formation in a lethal intraabdominal sepsis model in mice.