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There is no correlation between the minimal inhibitory concentration of teicoplanin against Staphylococcus epidermidis and efficacy in the mouse sepsis/peritonitis model

Abstract number: 1134_04_102

Batard E., Juvin M.E., Jacqueline C., Potel G., Drugeon H.

Objectives:  

The predictive value of teicoplanin Minimal Inhibitory Concentration (MIC) for clinical efficacy in the treatment of Staphylococcus epidermidis infections has been questioned. We aimed to assess the relation between the MIC and experimental in vivo efficacy of teicoplanin against Staphylococcus epidermidis.

Methods:  

We selected 7 clinical strains of Staphylococcus epidermidis with MICs of teicoplanin ranging from 0.25 to 32 mg/L. Two ciprofloxacin-resistant mutants (strains 15657 and A1894) were respectively obtained from wild-type strains 7453 and 7318. MICs were determined by agar dilution. Cyclophosphamide pre-treated female 20-gram Swiss mice were inoculated intraperitoneally with 2 107 CFU. Antibiotics (teicoplanin or ciprofloxacin, used as a control antibiotic) were administered subcutaneously at increasing concentrations immediately and 4 h after inoculation. Median effective doses (ED50) were calculated from mortality at day 6. Correlation was tested between log2 transformed MIC and ED50.

Results:  

MICs and ED50s are reported in the table. There was a significant positive correlation between log2 MIC and ED50 for ciprofloxacin (r2 = 0.79, p = 0.005), but not for teicoplanin (r2 = 0.35, p = 0.18).

StrainCiprofloxacin MIC (mg/L)Ciprofloxacin ED50 (mg/kg)Teicoplanin MIC (mg/L)Teicoplanin ED50 (mg/kg)
74530.12514.50.2545.6
73180.1252.30.52.3
7026118423.2
74182>50324.8
73714>50161.9
156578>50Ndnd
A18948>50Ndnd
533132>5045.3
5056Ndnd41.5

Conclusion:  

Our data suggest a lack of correlation between MIC of teicoplanin and in vivo activity in the mouse sepsis/peritonitis model. Further experimental and clinical studies are needed to determine the impact of elevated MICs of teicoplanin against Staphylococcus epidermidis.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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