Fusidic acid impregnation inhibits adherence of S. epidermidis to peripheric venous catheters

Abstract number: 1134_04_100

Agalar C., Kilic D., Ceken S., Ozturk E., Denkbas E., Kaygusuz S.

Objective:  

To evaluate the effect of impregnation of fusidic acid on adherence of slime poisitive S. epidermidis to peripheral catheters in an in vitro model.

Methods:  

Segments of 0.5 cm of uncoated catheters were cut. Both internal and external surfaces of these catheters were coated with fusidic acid dispersed in polylactic acid/dichloromethane solution. Fusidic acid release experiments were performed under unstirred conditions at pH 7.4 as a physiological buffer. Fusidic acid-impregnated catheters were added to the release medium. At predecided intervals, samples were withdrawn and the release of fusidic acid in the buffer solution was followed by UV spectrophotometer at 250 nm. The cumulative fusidic acid released into the medium from catheters was measured as a function of time. Fusidic acid impregnated and naive cahteters were incubated in Brain Heart Broth containing 104 cfu/ml slime positive S. epidermidis. At intervals of 12, 24, 48, 72 hours catheters were washed and vortexed for two minutes in 1 ml of physiologic saline. 100 microliter of samples of vortexed material and Brain Heart Broth were incubated in blood agar. 24 hours later colony numbers were assessed.

Results:  

Growths at 12, 24, 48, 72 hours were, 104, 105, 105, 105 colony/ml, and 10, 0, 0, 0 colony/ml, in naive catheters and impregnated catheters, respectively. No bacteria was cultivated from the Brain Heart Broth containing fusidic acid impregnated catheters 24 hours afterward.

Conclusion:  

Adherence of slime positive S. epidermidis to catheter in in vitro conditions was restricted with fusidic acid impregnation.