Occurrence of resistance to wide spectrum of antimicrobial agents in clinical isolates of the genus Acinetobacter

Abstract number: 1134_03_205

Sodomova E., Michalkova-Papajova D., Vrabelova-Poczova M., Rovna D., Kettner M.

Objectives:  

The aim of the study was to determine the occurrence of resistance to 33 antimicrobial agents in Acinetobacter clinical isolates.

Methods:  

Clinical isolates included in the study originated from Hospital Ruzinov, Bratislava (Slovakia). The majority of 37 isolates tested were obtained from Burn Department (35.1 %) and from Surgical Department (29.7 %) of this hospital and were isolated from burn infections (29.7 %) and wounds (27.0 %). The isolates were identified by NEFERMtest24 (Pliva-Lachema, Czech Republic) and selected as resistant to clinically used beta-lactam antibiotics. Resistance to antimicrobial agents was determined by standard disk diffusion method according to the NCCLS recommendations. The following antimicrobials were tested: mezlocillin, ticarcillin, piperacillin, carbenicillin, ampicillin-sulbactam, piperacillin-tazobactam, ticarcillin-clavulanic acid, ceftazidime, cefepime, cefoperazone, cefotaxime, ceftriaxone, ceftizoxime, moxalactam, imipenem, meropenem, aztreonam, gentamicin, amikacin, tobramycin, netilmicin, tetracycline, doxycycline, minocycline, ciprofloxacin, levofloxacin, lomefloxacin, norfloxacin, ofloxacin, gatifloxacin, chloramphenicol, trimethoprim-sulfamethoxazole, sulfonamides.

Results:  

In the set of 37 clinical isolates 100.0 % were resistant to mezlocillin, 86.5 % to ticarcillin, 100.0 % to piperacillin, 100.0 % to carbenicillin, 10.8 % to ampicillin-sulbactam, 43.2 % to piperacillin-tazobactam, 32.4 % to ticarcillin-clavulanic acid, 56.8 % to ceftazidime, 21.6 % to cefepime, 100.0 % cefoperazone, 75.7 % to cefotaxime, 75.7 % to ceftriaxone, 86.5 % to ceftizoxime, 75.7 % to moxalactam, 0.0 % to imipenem, 0.0 % to meropenem, 59.5 % to aztreonam, 100.0 % to gentamicin, 62.1 % to amikacin, 10.8 % to tobramycin, 5.4 % to netilmicin, 100.0 % to tetracycline, 54.1 % to doxycycline, 45.9 % to minocycline, 100.0 % to ciprofloxacin, 78.4 % to levofloxacin, 100.0 % to lomefloxacin, 100.0 % to norfloxacin, 43.2 % to ofloxacin, 83.8 % to gatifloxacin, 100.0 % to chloramphenicol, 59.5 % to trimethoprim-sulfamethoxazole and 86.5 % to sulfonamides.

Conclusions:  

More than 75 % of the clinical isolates were resistant to 20 or more from 33 antimicrobial agents tested. Carbapenems (imipenem and meropenem) were the only antimicrobial agents effective to all clinical isolates.