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The patterns of nasopharyngeal microflora in pre-school children with recurrent respiratory tract infections

Abstract number: 1134_03_7

Kosikowska U., Korona-Glowniak I., Los R., Biernasiuk A., Malm A.

Objectives:  

Respiratory tract infections, predominantly of viral etiology, are the most common, community-acquired infections in young children. Some of them are complicated by bacterial infections usually of endogenous origin. The mucous membranes of nasopharynx are known to be the important reservoir of some opportunistic or potentially pathogenic bacteria. The aim of the present study was to compare the nasopharyngeal microflora in two groups of pre-school children – without or with the recurrent respiratory tract infections.

Methods:  

Nasal and throat specimens were obtained from 225 children aged 3–5 years. The cotton swabs were immediately placed onto appropriate nonselective (blood agar) or selective media (Haemophilus chocolate agar, Chapman agar, McConkey agar or Sabouraud agar). Plates were incubated in an appropriate atmosphere – with or without increased CO2 concentration) for 18–48 hrs at 35°C. The isolated microorganisms were identified on the basis of routinely methods (macroscopic, microscopic or biochemical assays) or by rapid commercial latex tests – Slidex Staph-Kit and Slidex Pneumo-Kit (bioMerieux).

Results:  

The prevalence of potentially pathogenic bacteria typical for nasopharynx such as Staphylococcus aureus, Streptococcus pneumoniae, Moraxella catarrhalis or Haemophilus influenzae and also of yeast-like fungi (Candida sp., mostly C. albicans) was similar in both groups of children. However, in nasopharynx of children with the recurrent respiratory tract infections several opportunistic bacteria belonging to Enterobacteriaceae (Escherichia coli, Citrobacter freundii) or non-fermentative rods (Pseudomonas putida, Agrobacterium radiobacter, Acinetobacter lwoffii) were found. Also other bacteria such as slime-producing Bacillus sp. or streptomycetes were isolated from this group of children.

Conclusion:  

The obtained data suggest that young children with the recurrent respiratory tract infections are predisposed for nasopharynx colonization by several opportunistic bacterial species of Gram-negative rods or even by some unusual microorganisms, e.g. slime-producing Bacillus sp. or streptomycetes.

Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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