The performance of the new streptococci panel for BD Phoenix
Abstract number: 1134_01_312
Schülin T., Bochem E., Voss A.
Identification of streptococci (beta-hemolytic and viridans streptococci) can cause problems with conventional biochemical test or identification kits. We investigated the performance of a new streptococci identification panel for the automated system BD Phoenix.
Ninety-two clinical isolates of streptococci from sterile body sites were investigated. Thirty-nine were beta-hemolytic streptococci (Lancefield group A, B, C, G), 17 pneumococci, and 36 viridans streptococci. Reference methods were latex agglutination for beta-hemolytic streptococci, optochine susceptibility and bile-solubility for pneumococci and 16S sRNA and sequencing for all other streptococci, including some pneumococci. The Phoenix panels were inoculated from a fresh culture and processed as advised by the manufacturer.
Seventy-seven (84%) strains were identified correctly, including all beta-hemolytic streptococci, 12 (13%) strains were identified incorrectly, and 3 (3%) strains could not be identified at all by the system. Only one third (4/12) of the S. mitis strains, which is an important pathogen in hematological patients, were identified correctly.
Beta-hemolytic streptococci and pneumococci were reliably identified by the automated system. Identification of viridans streptococci esp. S. mitis is less reliable. Therefore further attention is needed to better identify viridans streptococci.
|Session name:||XXIst ISTH Congress|
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