The first case of haemolytic-uraemic syndrome caused by STEC O26 in Slovakia
Abstract number: 903_r2291
Shiga toxin-producing Escherichia coli (STEC) are associated with life-threatening sequelae such as haemolytic-uraemic syndrome (HUS). Particularly STEC O157 appears to be the most frequent. However, it has been recognised for a number of years that STEC belong to a broad range of O serogroups. We report a case of HUS caused by Stx2-positive STEC O26.
Girl (4 years) was admitted to Hospital for Sick Children with signs of HUS. Stool samples from patient and her family living in the same house were collected. There were used selective media (CT-SMAC, Enterohaemolysin agar, Oxoid, UK) for STEC detection, immunomagnetic separation with anti-O157 beads (Dynal, Norway), Vero cell assay and latex agglutination detecting shiga toxin 1 and 2 (Stx1,2) (Oxoid, UK), multiplex PCR using specific primers for genes encoding Stx1, Stx2, enterohaemolysin and intimin. Epidemiological information was obtained by semi-structured interviews undertaken by Epidemiology Unit staff with all family members and contacts.
On the basis of collected samples we have isolated sorbitol-fermenting Escherichia coli O26 strain. Isolate originated from patient with HUS. Isolate harboured the stx2 gene, the eae gene encoding intimin and the ehxA gene encoding enterohaemolysin. Stx2 activity was also demonstrated. Stool samples taken from family members were negative for STEC O26.
Our data indicate the first documented case of HUS caused by shiga toxin 2-producing Escherichia coli O26 in our geographical area."
|Session name:||XXIst ISTH Congress|
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