Genomic variability of pre-S/S region of hepatitis B virus from patients in the Czech Republic
Abstract number: 903_r2288
For global epidemiology of hepatitis B virus both phenotype variability i.e. HBsAg subtypes and genotype variability are used. For determination of routes of transmission in local outbreaks, comparison and analysis of nucleotide sequences of HBV DNA from individual patients is needed. The knowledge of heterogenity of HBV genome in patients from given geographic region is useful. The aims of this study was to determine heterogenity of pre-S/S region of HBV in the Czech Republic and to use this knowledge for analysis of local outbreak of HBV infection among patients of a haemodialysis unit.
HBV pre-S/S region DNA amplified by PCR from sera of 182 hepatitis B patients was sequenced. Consensus nucleotide sequences were obtained using CLUSTAL W (European Bioinformatics Institute, GB) followed by manual editing using Bioedit 5.09. Phylogenetic trees were constructed with MEGA 2.1 using UPGMA Kimura two-parameter model.
HBV-DNA was amplified by PCR from sera of 166 patients from different regions of the Czech Republic. Part of pre-S/S region from PCR products were sequenced. Nucleotide sequences were aligned and phylogenetic tree was constructed. In the tree were found 14 pairs of identical sequences, one cluster with three identical sequences, one cluster with four, one with five and one with 12 identical sequences. These 18 clusters were analysed from geographic point of view and retrospectively affordable data about epidemiological relatedness of cases were considered. Fourteen clusters were found in the group of patients with genotype A, three clusters in patients with genotype D and one cluster in the group with genotype B. The new phylogenetic tree was constructed when nucleotide sequences from 16 patients infected with HBV during the outbreak in a haemodialysis unit were added. The source of infection and route of transmission of HBV in this outbreak was identified with contribution of phylogenetic analysis.
This study provided data about heterogenity of the part of HBV genome-coding surface antigen among HBV infected patients in the Czech Republic. Study confirmed that sequence analysis of pre-S/S region of HBV DNA is useful epidemiological tool for identification of routes of transmission of HBV infection, mainly in medical facilities.
Supported by grant IGA MZCR No. NI 6796-3."
|Session name:||XXIst ISTH Congress|
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