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Heterologous expression of the HPV16.E6 increases the resistance to stress

Abstract number: 903_r2251

Pinto Oliveira A.

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The malignant phenotype of high-risk types human papillomavirus depends mainly on the expression of two viral genes, E6 and E7. If control of E6 is lost, this protein can trigger immortalisation of cells and cancer.

The aim of this study is to determine if E6 protein changes the response of yeast to stress, as a model of eukaryotic cell immortalisation.

In the present work we expressed HPV16 E6 (pYES2.E6) in S. cerevisae in order to study the response to thermal stress, to pheromone recovery, and to caffeine treatment. The heterologous expression of E6 in yeast results in a higher resistance towards caffeine, but there are no changes when the cells are incubated in high temperatures. In what regards pheromone treatment, E6 expressing cells are able to overcome the cell cycle arrest, albeit exhibiting the morphogenetic alterations due to the presence of pheromone. Since the protein kinase C is a central protein in the general response to stress a deleted mutant in PKC1 was transformed with pYES2.E6, in order to study the suppression of the increased resistance to stress.

The subcellular localisation of the protein E6, under stress conditions, is studied by co-localisation of the fusion protein E6-GFP, using an inducible (pYES2) and a constitutive (pUG35) system.

The results obtained points to the conclusion that the heterologous expression of E6 in the yeast S. cerevisiae leads to an increased resistance to stress, showing that E6 interacts with specific components of the biochemical machinery of yeast cells.

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Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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