Objectives:

In our university hospital, the chromogenic media recently started to be integrated and used in parallel with Columbia 5% sheep blood, Cled and Mac Conkey agar. We have evaluated the fertility, identification performance for the microorganisms isolated in our laboratory and the enumeration speciality of the new media called CPS ID 3 (bioMérieux S.A. France).

Methods:

In total, 105 urine specimens were included in this study, collected between September and November 2003 in the university hospital of Kahramanmaras Sutcu Imam. The specimens were inoculated in parallel on the CPS ID 3 medium, Columbia 5% sheep blood, Cled and Mac Conkey agar. They were incubated at 37 °C during 18–24 h.

Results:

Seventeen specimens were sterile (<1000 CFU/mL), they were not included in the study. 65 of 79 specimens had a single type colony, and 14 of 79 had mixed type colony (12 of 14 two types, two of 14 three types). The distribution of the bacteria were respectively: 44 Escherichia coli, 10 Enterobacter spp., 8 Proteus spp., 8 Klebsiella spp., 3 Pseudomonas aeruginosa, 4 B group streptococci, 7 Enterococcus spp., 6 Staphylococcus spp, 2 Candida spp. and 2 Morganella spp.

The sensitivity of CPS ID 3 for identification of E. coli,Klebsiella, Enterobacter, enterococci were 93.2, 100, 100 and 100%, respectively. CPS ID 3 efficiently prevented the swarming of Proteus.

Conclusion:

CPS ID 3 is a useful chromogenic media to perform urine specimen testing because of rate of identification of the most frequent bacteria, efficient enumeration and fertility.