Bacterial contamination and drug resistance in isolated bacteria from intensive care units (ICU and NICU) in Hamadan hospitals, West Iran

Abstract number: 903_r2062

Yousefi Mashouf R.


Many reports from all over the world indicate that multi-drug resistance in nosocomial organisms are increasing. Bacterial contamination in intensive care units, surgery and burning rooms is also one of the major problems in hospitals. The aims of this study were determination of bacterial contamination and antibacterial drug resistance in isolated bacteria from intensive care units (ICU and NICU) in Hamadan hospitals (Mobasher Kashani and Fatemie).


In this study, 682 samples were randomly collected from different areas including devices and apparatus, physical surfaces and staff uniforms. The samples were inoculated in EMB and blood agar by wet swabs and transferred to medical laboratory for identification and antibiogram of strains. A total of 140 cases were selected and cultured on Muller–Hinton agar for antibiogram tests (Kirby–Bauer method). The antibiotics disks consisted of: erythromycin, vancomycin, ciprofloxacin, carbenicillin, penicillin G, tetracycline, chloramphenicol, ampicillin, gentamycin and ceftriaxone.


The ICU of Mobasher Kashani hospital showed more bacterial contamination (57.4%) rather than the NICU of Fatemie hospital (48.3%). The most bacteria isolated from two hospitals were as follow: micrococci, Staphylococcus epidemidis, Staphylococcus aureus, Pseudomonas aeroginosa, E.coli, Klebsiella, Enterobacter, Proteus, Acinetobacter and Streptococcus faecalis. The most contaminated part (80.3%), were the sink and staff uniforms the ICU of Mobasher Kashani hospital. In this study, more than 80% of strains which isolated from the ICU of Mobasher Kashani hospital were resistant to: erythromycin, vancomycin, carbenicillin, tetracycline, chloramphenicol and ampicillin, whereas, the strains of the NICU of Fatemie hospital were resistant to penicillin, ampicillin, chloramphenicol and gentamycin.


Our results showed that intensive cares of Hamadan hospitals both the ICU of Mobasher Kashani and the NICU of Fatemie were contaminated with routine nosocomial microorganisms. There were also the high drug resistance (in vitro) in strains isolated from two hospitals.


It seems that sterilisation and disinfection methods in hospitals were not performed correctly and related authorities should control it regularly.


Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Location: Oxford, UK
Presentation type:
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