Objectives:

In recently years, antifungal drug resistance has dramatically been increased with resulted in the unsuccessful intreatment of dermatophytic patients. Thus, the emergency of finding new antifungal drugs with low side-effect has recently been noted by several researchers. Fungal infection of the skin and its appendages specially dermatophytosis to be considered as the most prevalent skin disorders. Dermatophytosis is a fungal infection which involve the stratum corneum of skin, hair and nail. This contagious animal infection caused by a special group of fungi called dermatophytes.

Methods:

In this study the effect of different concentrations of two known antifungal drugs, griseofulvin (8.25–2000 mg/mL) and terbinafine (0.005–4mg/mL) on Microsporum canis, Epidermophyton floccosum, Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum investigated. The fungal isolates were separately added to different concentrations of above-mentioned drugs (1000 cells/mL) and then mixed with semisolid SDA medium in plates. Then the plates were incubated at 28^[compfn]C for 7–12 days. The drug free sample was mentioned as control for MIC (minimum inhibitory concentration) and MFC (minimum fungicidal concentration).

Results:

The results show that the MIC, 50–100% for both antifungal drugs. This growth for inhibitory effects of griseofulvin was obtained at 1–31.25, 1–31.25, 2–125, 0.06–0.25 and 0.03–0.25 mg/mL for Microsporum canis, Epidermophyton floccosum, Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum, respectively. These values for terbinafine were measured as 0.007–0.5, 0.007–0.125, 0.005–0.5, 0.06–0.25 and 0.03–0.25 mg/mL for M. canis, E. floccosum, M. gypseum, T. mentagrophytes and T. rubrum accordingly.

Conclusions:

In spite of difference observed in antifungal susceptibility, the examined fungi our results indicated that both drugs can effectively inhibit the growth of some important native dermatophytes and thus, have potential values for treatment of clinical dermatophytosis in human and animals.