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Characterisation of integrons carrying blaVIM type metallo-beta-lactamase genes from carbapenem-resistant Pseudomonas aeruginosa clinical isolates in Hungary

Abstract number: 902_p1877

Libisch B.

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Objectives:

The aim of this study was to investigate the presence and frequency of occurrence for acquired metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa clinical isolates in Hungary.

Methods:

We screened the isolates by EDTA-inhibition based phenotypic tests together with PCR using integron, blaVIM and blaIMP specific primers to identify acquired MBL-producers.

Results:

We recently isolated the first integron-borne MBL gene in Hungary from the urine sample of a Greek citizen treated in August 2002 in Budapest. DNA sequencing revealed the presence of a Class 1 integron carrying three gene casettes: an OXA-type lactamase, an aminoglycoside 6'-N-acetyltransferase and a blaVIM-4 type MBL gene cassette. VIM-4 has been reported for the first time from Greece in 2002. These observations raise the possibility that this P. aeruginosa strain originates in Greece, and has been imported to Hungary by a human carrier. In October 2003 another VIM-producing P. aeruginosa strain has been isolated in Southern Hungary. PCR mapping experiments suggest that this strain carries a class 1 integron with only two resistance gene cassettes in its variable region. The blaVIM type MBL gene cassette is right upstream of the 3' conserved sequence. The structural diagram of the two integrons is presented with implications for their possible phylogenetic relationship.

Conclusions:

With the isolation of a second VIM-producing strain from an apparently independent case the repeated appearance of MBL producing clinical isolates can be anticipated in Hungary. A regular screening/monitoring system should be set up to prevent the wider spread of these resistance determinants.

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Session Details

Date: 01/08/2007
Time: 00:00-00:00
Session name: XXIst ISTH Congress
Subject:
Location: Oxford, UK
Presentation type:
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