In recent years, cutaneous tuberculosis (CTB) with an atypical clinical appearance has become more common in developing countries and is often confused with other granulomatous dermatosis. The diagnosis of CTB remains troublesome, because of the difficulty of detecting mycobacteria in skin lesions by conventional laboratory methods. Lupus vulgaris (LV), the commonest of all forms of CTB can affect earlobes as well as the other parts of the body. In this article, we present a 20-year-old male patient with LV of left earlobe. In the initial period, pyodermia was the misdiagnosis and the patient was treated superfluously with antibiotics for four years, elsewhere. The definitive diagnosis of the disease was confirmed by polymerase chain reaction (PCR). Mycobacteria could not be seen or isolated by stained smears and conventional and radiometric culture methods from the skin biopsy specimens. The lesion was treated successfully with antituberculous chemotherapy (ATBC). PCR is a reliable and a very rapid method for establishing or confirming the diagnosis of CTB as seen in our patient.